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KMID : 0361019990420010039
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Korean Journal of Otolaryngology - Head and Neck Surgery
1999 Volume.42 No. 1 p.39 ~ p.46
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Effect of Retinoic Acid on the Differentiation and Lysozyme Expression in Human Airway Epithelial Cells
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윤주헌/Joo Heon Yoon
김경수/이호기/김성식/홍성수/조성우/이정권/박인용/Kyung Su Kim/Ho Ki Lee/Sung Shik Kim/Seong Soo Hong/Sung Woo Jo/Jeung Gweon Lee/In Yong Park
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Abstract
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Background and Objectives: Retinoic acid (RA)-deprived cultures of normal human tracheobronchial epithelial (NHTBE) cells became squamous metaplastic, failed to produce mucin and instead secreted or released large amounts of lysozyme (LZ).
The
purpose of this study was to elucidate the relationship between RA-deficiency induced squamous metaplasia and increased LZ as a function of time. Materials and Method: The change of lysozyme protein and lysozyme mRNA was investigated over
time in
cultures using passage-2 normal human tracheobronchial epithelial (NHTBE) cells and passage-2 normal human keratinocytes (NHK). The amount of lysozyme and mucin was measured with dot blot, message of lysozyme with RT-PCR, and cornifin mRNA with
Northern
blot. Results: Lysozyme message levels were consistently higher in RA-sufficient than RA-deficient cultures. Intracellular and extracellualr LZ increased to a peak on the day 16 and thereafter decreased in the RA-deficient cultures. LZ gen
expression in the RA-deficient cultures was barely detectable on the day 7 but was clearly expressed between days 10 and 14, but thereafter message levels decreased markedly. On day 12, large numbers of cells began to exfoliate in the
RA-deficient
cultures. Extracellular LZ appeared simultaneously at the apical surface, presumably released from the exfoliated cells, which contained high concentrations of LZ. Intracellular LZ levels were more than 11 fold less in NHK cells compared to NHTBE
cells.
Conclusion: This study suggests that cellular accumulation of lysozyme protein is a unique feature of metaplastic squamous differentiation. Further studies are needed to find out what mechanisms are involved.
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KEYWORD
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